title: Table of Contents

text:
Table of Contents
The Importance of Alloy Wheels in Modern Automobiles
Alloy wheels have become a popular choice for modern automobiles, offering both aesthetic appeal and functional benefits. Unlike traditional steel wheels, alloy wheels are made from a mixture of metals, typically aluminum, which provides a lighter and stronger alternative. This design yields significant advantages in performance, handling, and fuel efficiency.
One of the primary benefits of alloy wheels is their reduced weight compared to steel wheels. A lighter wheel means less unsprung mass, allowing for improved suspension response. This enhances the vehicle’s overall handling, making it more agile and responsive to driver inputs. As a result, drivers can experience a more dynamic driving experience, especially during cornering.
In addition to performance benefits, alloy wheels also contribute to improved fuel efficiency. Since they are lighter, vehicles equipped with alloy wheels require less energy to move, which can lead to better gas mileage. This is particularly important in an era where fuel economy is a significant concern for many car owners. By opting for alloy wheels, drivers can enjoy both style and savings at the pump.
The aesthetic appeal of alloy wheels cannot be overlooked. Available in various designs, colors, and finishes, they allow car owners to personalize their vehicles and enhance their overall look. Whether it’s a sleek silver finish or a bold black matte, alloy wheels can complement any car’s design and elevate its visual impact.
Furthermore, alloy wheels are often easier to maintain than traditional steel wheels. Their surfaces can resist corrosion and rust better, which is particularly beneficial in regions that experience harsh weather conditions. With proper care, alloy wheels can maintain their luster for years, contributing to the vehicle’s longevity and resale value.
Choosing the Right Alloy Wheels
When selecting alloy wheels for your vehicle, there are several crucial factors to consider. First and foremost, it is essential to ensure that the wheels are compatible with your car’s specifications. This includes checking the bolt pattern, offset, and diameter to guarantee a proper fit.
Another important aspect to consider is the wheel’s finish. Alloy wheels come in various finishes, such as polished, painted, or anodized. Each finish offers different levels of durability and aesthetic appeal, so it’s vital to choose one that aligns with your preferences and lifestyle. For instance, a polished finish may offer a stunning shine but might require more maintenance than a painted option.
It’s also worth noting the intended use of the vehicle when choosing alloy wheels. For example, if you frequently drive on rough terrain or off-road conditions, investing in wheels designed for rugged environments is advisable. These wheels are built to withstand harsh impacts and provide additional strength and durability.
Additionally, consider the wheel size. Larger wheels can enhance the car’s appearance and improve handling but may lead to a stiffer ride. Conversely, smaller wheels often provide a smoother ride but may not deliver the same level of performance. Therefore, it’s crucial to find a balance between aesthetics and functionality that suits your driving style.
Finally, don’t overlook the importance of quality. When it comes to alloy wheels, investing in reputable brands is essential. High-quality wheels are not only more durable but also safer, reducing the risk of damage during everyday driving or challenging conditions.
Maintenance Tips for Alloy Wheels
Serial Number | Product Name |
1 | Wheels |
Maintaining alloy wheels is vital to ensure their longevity and keep them looking their best. Regular cleaning is the first step in proper maintenance. It is advisable to wash alloy wheels frequently using a pH-balanced wheel cleaner, as harsh chemicals can damage the finish.
In addition to regular cleaning, it’s essential to inspect the wheels for any signs of damage, such as curb rash or cracks. Early detection allows for timely repairs, preventing further damage and preserving the wheel’s integrity. If you notice any issues, consulting a professional is recommended to assess whether repairs are feasible.
Proper storage is another critical aspect of maintaining alloy wheels, particularly if you switch between seasonal tires. Storing wheels in a cool, dry place away from direct sunlight can prevent deterioration. Covering them with protective bags can also help shield them from dust and contaminants.
Tire pressure should be monitored regularly, as incorrect tire pressure can lead to uneven wear on both tires and wheels. Maintaining the recommended tire pressure ensures optimal performance and extends the life of your alloy wheels.
Lastly, consider applying a protective sealant on the alloy wheels to create an additional barrier against dirt, grime, and brake dust. This can make future cleaning easier and help maintain the wheel’s shine over time. Proper maintenance will not only enhance the appearance of your alloy wheels but also contribute to the overall performance and safety of your vehicle.


tier: cub

shelf: software-docs

title: phpMyAdmin in Website Hosting

text:
phpMyAdmin in Website Hosting
You shall be able to employ phpMyAdmin to access any database you set up in a website hosting account purchased from our company as we supply the tool by default with all packages. As soon as you log in to your Hepsia hosting CP and create a new database in the MySQL Databases section, a small phpMyAdmin icon shall appear on its right-hand side. All it takes to log in automatically is to click on that icon and the tool will open in a new Internet browser tab, so that you can handle the database data. That is also the way to import a database if you are moving your web site from another host company to our state-of-the-art cloud hosting platform. A different way to log in to a database is if you take advantage of our direct phpMyAdmin login URL, but you need to know the DB account info. This option will enable you to give access to any database to other people, without giving them access to the whole web hosting account.
phpMyAdmin in Semi-dedicated Hosting
phpMyAdmin is one of the software tools which come with all our Linux semi-dedicated hosting. You may log into it and handle all of your MySQL databases if you click on the phpMyAdmin icon located on the right side of each and every database which you have created through your Hepsia hosting Control Panel. You will not have to do anything else, because our system will log you in automatically and you can easily proceed with the tasks you should do - import a database file from another company, change some content, and so on. You could access a database by using the direct phpMyAdmin login page too, but in this case you will have to type in the correct account information. You could use this option if you hire a website designer, as they can work on your Internet site without accessing your website hosting Control Panel. Thus, all files and e-mails which you have in the account will be secure.


shelf: health

title: Testimonials

text:
Read what our clients say about our office and patient care. We're proud of our 5 star reviews and the reputation we've established in the community.
"I went to TSO to get an updated prescription. Everyone here was friendly and helpful, and the doctor took her time to explain what each part of the eye exam meant for my eye health. I will return to this TSO location for any future needs."
"Dr. Dennington is wonderful, professional, highly skilled in her trade excellent bedside manner and I could go on and on.
I highly recommend her to everyone."
"Excellent and knowledgeable Doctors and staff. I was seen at the exact time of my appointment, with no wait. Great experience!"
"Very knowledgeable and caring professionals. ❤ Dr Dennington and her office personnel."
"Nice and clean place. Dr. Dennington took her time and answered all of my questions and even got a second opinion with another doctor in office. I really appreciated her thoroughness will definitely be back!"
"The best eye Dr around!! Phenomenal staff and service! Beautiful and clean office! Dr. Dennington is the best! Highly recommend!!"
"best first experience ever! brought our 5 year old in and she’s very anxious (and hyper) at doctor appts - from start to finish the staff treated us with kindness and lots of patience! we will definitely be back"
"Awesome! Dr. Dennington and all the staff were professional, knowledgeable, and keep a welcoming environment. My eye exam was very thorough, no outdated equipment, and listened to and addressed my individual concerns and questions about eye health and glasses."
"Best eye exam experience I’ve had. Doctor and staff were so friendly and made me feel comfortable all the way through. I highly recommend you give them a try!"
"Dr. and staff are wonderful. This is the second time I've had a semi-emergency and both times they have worked me in quickly and they couldn't have been nicer"


shelf: finance

text:
Independent Research Data Shows Franchisees Are Highly Satisfied with Payroll Vault’s Innovation and Creativity
Littleton, Colorado, October 10, 2022 – Payroll Vault was identified by Franchise Business Review as one of just 100 franchise brands to be named an award-winning franchise in its 2022 report on the Most Innovative Franchises.
A niche for a franchise opportunity, Payroll Vault Franchising is helping entrepreneurs build a legacy business and professional lifestyle in a rapidly growing industry. The Payroll Vault brand continues to amplify with strength and momentum.
Franchise Business Review, a research firm that performs independent surveys of franchisee satisfaction, provides the only ratings of franchises based solely on actual franchisee satisfaction and performance. Franchise Business Review publishes annual rankings of the top franchises in its Guide to Today’s Top Franchises, as well as guides throughout the year ranking the top franchises in specific sectors.
To identify the companies on the list of this year’s Most Innovative Franchises, Franchise Business Review analyzed 18 months’ worth of data from over 30,000 franchisees representing more than 300 brands. Franchise owners were asked about their brand’s innovation and creativity as well as their trust in the franchisor, overall satisfaction with the brand, and likelihood to recommend it to others.
“Innovation takes a lot more than just having creative ideas and solutions. It also takes execution,” said Eric Stites, founder and CEO of Franchise Business Review. “Each of the companies on our list of the Most Innovative Franchises has developed and implemented products, processes, or services that enable and sustain growth and long-term relevancy. We are proud to recognize the franchises that prioritize and value innovation to support their franchisees’ success and the long-term resiliency of the brand.”
Payroll Vault’s franchisees were surveyed on 33 benchmark questions about their experience and satisfaction regarding critical areas of their franchise systems, including leadership, training & support, operations, franchisor/franchisee relations, and financial opportunity.
Sean Manning, Founder and CEO of Payroll Vault Franchising stated, “Innovation often creates a vision of technology, but innovation is also closely linked to systems, methodologies, and new business relationships under a clear vision. We continuously evaluate and upgrade our processes and systems as we identify efficiencies. The goal is to continue to provide a strong entrepreneurial opportunity to emerging and advanced businesspeople while redefining the payroll experience.”
Visit FranchiseBusinessReview.com to see the full list of the 2022 Most Innovative Franchises.
About Payroll Vault Franchising
Payroll and Workforce Management services are growing requirements for small businesses. Payroll Vault Franchising offers the opportunity to own a boutique-style, full-service payroll company supported by a team of local experts and a nationally recognized brand. From the franchise launch in 2012, Payroll Vault Franchising has rapidly grown because of exemplary client service and is an industry leader receiving numerous accolades and awards nationally. For more information, visit www.PayrollVault.com/franchise.com.
About Franchise Business Review
Franchise Business Review (FBR) is a leading market research firm serving the franchise sector. FBR measures the satisfaction and engagement of franchisees and franchise employees and publishes various guides and reports for entrepreneurs considering an investment in a franchise business. Since 2005, FBR has surveyed hundreds of thousands of franchise owners and over 1,200 leading franchise companies. FBR publishes free and unbiased franchisee satisfaction research reports throughout the year online at http://www.FranchiseBusinessReview.com. To read our publications, visit https://franchisebusinessreview.com/page/digital-guides/.
Chief Marketing Officer
Franchise Business Review


shelf: education

title: Sprachaufenthalt englisch erfahrungen mk

text:
The main reason was that I wanted to work as a volunteer in the Timion project in South Africa. Although I had already attended English courses in Switzerland, I had not yet been able to put the theory into practice. I have an education in the export sector, which is why I wanted to improve my English as a secondary reason.
I was able to spend my language study trip in Cape Town, South Africa.
The first day at school started on September 5, 2016. The six invested weeks flew by very quickly.
What surprised me was that the students almost always spoke to each other in English. We Swiss also did a lot together and almost always spoke English. The language school didn't just motivate the students to do homework, they motivated us to learn to love this language. To sum up, I am very happy that I chose this place.
I was able to move up from level B1 to B2, which helped me a lot for my subsequent volunteer placement. As soon as I return to Switzerland, it will be easier for me to find a job in my field and I will be able to ask for a bit more pay.
I recommend a host family, as you have additional language training every evening. You also know a local who knows the language and who you can ask anything you want, and you have a place where people cook and socialize. Next time, I would invest at least eight weeks in the language study trip, as six weeks was rather short.
I registered with IH School Cape Town through Surprise Reisen.
IH School Cape Town has very motivating teachers and is located in Seapoint. In my opinion, this is the best location in Cape Town, as the crime rate is very low, nature is very close and the school is almost directly by the sea. I am very satisfied and would recommend IH School Cape Town to others. Surprise Reisen also did a good job and was a reliable point of contact in Switzerland. If something had gone wrong, I would have been glad to have such a contact behind me.


shelf: home-craft

title: Fionas gubbeen potato farls

text:
Our former colleague and friend Fiona created these farls featuring Gubbeen in honour of St Patrick's Day.
Nothing hits the breakfast mark the day after a celebration than a hot potato farl!
As I type, I confess to actually being a Celtic cousin. As a Scot, I grew up on the ‘tattie scone’ and my lovely, late Mum Anne made these from leftover mashed potatoes (which had been binded with equal amounts of flour, love and more butter!).
I would have used her recipe if she had ever written it down but her brilliance and knowledge was always in her head. “I don't know how much flour I use!” she always explained, “it is how the mixture feels!”.
So the recipe below is by the brilliant Henry Dimbleby & Jane Baxter from ‘The Guardian,’ many farls ago. I added some grated Gubbeen as its savoury nuttiness and beautiful pink rind elavates your farl and is a joy to savour.
P.s. If you do have leftover mashed potatoes please channel your ‘inner Anne’ and give it a go! As to how much Gubbeen to add? I didn’t measure it, it was simply how the mixture felt!
INGREDIENTS (to make 8 small farls)
500g floury potatoes
50g plain flour, plus extra for rolling out
¼ tsp baking powder
Salt and black pepper
Extra butter, for cooking
1 Peel the potatoes, then cut them into quarters. Cover with water in a saucepan, season with a pinch of salt and cook for about 15 minutes or until they become tender. You can check this by sticking a knife into them, it should slide out again easily. Drain well, then return the potatoes to the pan.
2 Melt the butter in a pan and add to the potatoes. Mash until smooth. For the best results, pass the potatoes through a potato ricer or mouli – otherwise, use a standard potato masher.
3 Sift the flour with the baking powder into the potato mix and stir to combine. Add in some crumbled Gubbeen at this point for extra tastiness. Taste for seasoning and adjust, if required.
4 The dough should come together and away from the sides of the pan. You can add more flour if the mixture is too wet and a little milk if it is too dry.
5 Divide the dough into two halves. Form one piece into a ball, then roll it out on a floured surface and into a rough circle with a diameter of about 15 cm and a thickness of 5mm to 1cm. Cut the circle into quarters.
6 Heat a non-stick shallow frying pan over a medium heat with a little butter to grease the surface. Transfer the four pieces to the pan and cook for around 3 minutes or until golden brown. Flip over and cook the other side for another 3 minutes. Remove to a warm plate.
7 Repeat the process with the other half of the dough mix.
This recipe is adapted from 'The easy way to make farls' by Jane Baxter for The Guardian, March 2014.


shelf: history-culture

text:
Carnegie Hall Citywide: Orrin Evans and the Captain Black Big Band
Led by visionary pianist and composer Orrin Evans, the Grammy Award–nominated Captain Black Big Band delivers big-band jazz with bold energy and fearless creativity. Known for its explosive sound and improvisational fire, the ensemble transforms hard-swinging grooves, bluesy soul, and avant-garde edges into a thrilling, unpredictable ride. With Evans at the helm, expect a powerhouse performance that pushes boundaries and lights up the stage at this Citywide performance at Bryant Park.
The piano for this performance is provided with support by Steinway & Sons.
Carnegie Hall Citywide is the Hall’s free concert series that brings a diverse lineup of live performances to neighborhoods throughout New York City. Presented in partnership with esteemed community organizations across the five boroughs, the series highlights dynamic artists from a variety of musical genres—including classical, jazz, folk, Latin music, and more.
Bryant Park lends out hundreds of free blankets on a first-come, first-served basis, or bring your own cotton or fleece blanket. To protect the lawn, please do not sit on waterproof materials such as plastic tarps, yoga mats, or inflatable chairs.
Take a seat in one of the pre-set chairs on the lawn or grab a chair from the gravel. You can use a chair anywhere in the park except for the center lawn "blanket zone".
Bring your own picnic or purchase food and drinks from tents on the east side of the lawn. Attendees can enjoy cuisine from the five boroughs with a rotating line-up of artisanal vendors curated by Hester Street Fair.
Stout NYC also offers giant pretzels, gourmet popcorn and other light bites as well as a selection of beer, wine, and non-alcoholic beverages for purchase.
Visit the Bryant Park Shop tent for exclusive Picnic Performances merchandise and more!
Find a parachute, hula hoops, and more fun on the east side of the lawn at select events.
Dogs are welcome on the gravel and bluestone, but not on the lawn.
Bryant Park Picnic Performances presented by Bank of America is a free outdoor festival that welcomes all New Yorkers to experience the city’s vibrant arts and culture. The series provides a platform for extraordinary artists and serves as a vital outdoor venue for a wide array of New York’s cultural institutions.


shelf: civic-law

title: How do i file a discrimination charge

text:
Discrimination in the workplace should not be taken lightly and it is important to take action to combat this issue. Discrimination consists of mistreatment, denial of benefits or job, or harassment due to your gender identity, sexual orientation, race, religion, nationality, age, disability, genetic information, or sex.
The first step in handling an instance of employee discrimination is to consult with an experienced employment law attorney who can assess your situation and help you get started with your claim. If you believe that you have been discriminated against in your current or potential place of employment, you may want to file a Charge of Discrimination with the Equal Employment Opportunity Commission. You must file your charge within 180 days from the date that the discriminatory event took place. There are certain situations in which this deadline is extended to 300 days after the discrimination occurred. If it was a situation of ongoing discrimination with many events, the statute of limitations is 180 (or 300) days from the last harassment incident.
Once you have filed your charge, the EEOC may tell you to attend mediation sessions to try and solve the issue in a peaceful manner. However, mediation doesn’t always work and cases aren’t always sent to mediation. In that scenario, the EEOC will conduct an investigation to determine whether or not any laws were violated when the alleged instance of discrimination took place. If the investigation concludes a law was violated, a settlement may try to be reached. If there is no evidence suggesting unlawful behavior, you can obtain a Notice of Right to Sue, which will allow you to bring the case to court.
If you have questions about whether or not you may have a discrimination case, consult with an experienced employment law attorney who can assess your situation. Contact the Vigilante Law Firm, P.C. today.


tier: polar

shelf: science-engineering

text:
NASA's Chandra Observatory searches for trigger of nearby supernova
- August 16, 2014
- New data from NASA's Chandra X-ray Observatory offer a glimpse into the environment of a star before it exploded earlier this year, and insight into what triggered one of the closest supernovas witnessed in decades.
New data from NASA's Chandra X-ray Observatory offer a glimpse into the environment of a star before it exploded earlier this year, and insight into what triggered one of the closest supernovas witnessed in decades.
The data gathered on the Jan. 21 explosion, a Type Ia supernova, allowed scientists to rule out one possible cause. These supernovas may be triggered when a white dwarf takes on too much mass from its companion star, immersing it in a cloud of gas that produces a significant source of X-rays after the explosion.
Astronomers used NASA's Swift and Chandra telescopes to search the nearby Messier 82 galaxy, the location of the explosion, for such an X-ray source. However, no source was found, revealing the region around the site of the supernova is relatively devoid of material.
"While it may sound a bit odd, we actually learned a great deal about this supernova by detecting absolutely nothing," said Raffaella Margutti of the Harvard-Smithsonian Center for Astrophysics (CfA) in Cambridge, Massachusetts, who led the study. "Now we can essentially rule out that the explosion was caused by a white dwarf continuously pulling material from a companion star."
This supernova, SN 2014J, could instead have been caused by the merger of two white dwarf stars, an event that should result in little or no X-rays after the explosion. Further observations could rule out or confirm other possible triggers.
"Being able to eliminate one of the main possible explanations for what caused SN 2014J to explode is a big step," said CfA's Atish Kamble, a co-author of the study. "The next step is to narrow things down even further."
Type Ia supernovas are used as cosmic distance-markers, and have played a key role in the discovery of the universe's accelerated expansion. At about 12 million light-years from Earth, SN 2014J and its host galaxy are close -- from a cosmic perspective. This offers scientists a chance to observe details that would be too hard to detect in more distant supernovas.
"It's crucial that we understand exactly how these stars explode because so much is riding on our observations of them for cosmology," said co-author Jerod Parrent also from CfA. "SN 2014J might be a chance of a lifetime to study one of these supernovas in detail as it happens."
The study of SN 2014J is similar to a study led by Margutti about another supernova, SN 2011fe, in the nearby galaxy M101.
This study was conducted by CfA's Supernova Forensics Team, led by Alicia Soderberg. The results were published online and in the July 20 print issue of The Astrophysical Journal.
NASA's Marshall Space Flight Center in Huntsville, Alabama, manages the Chandra program for NASA's Science Mission Directorate in Washington. The Smithsonian Astrophysical Observatory in Cambridge, Massachusetts, controls Chandra's science and flight operations.
For an additional interactive image, podcast, and video on the findings, visit: http://chandra.si.edu
For a preprint of the study results in The Astrophysical Journal, visit: http://arxiv.org/abs/1405.1488
For Chandra images, multimedia and related materials, visit: http://www.nasa.gov/chandra
Cite This Page:


title: Post date: Oct 16, 2014 9:12:44 PM

text:
Post date: Oct 16, 2014 9:12:44 PM
IE - Windows - Inbox/Library drop-down menu functionality fixed
IE - Windows - Gear icon drop-down menu fixed
Added text selection highlighting
Add branded header to sent link landing pages - single record and playlist
When a user ‘Shares’ a link, the landing page displays the system default logo in the header. Note - if you have custom skins for different users, they will not see their custom skin logo on this landing page.
Fixed session handling to prevent partial page loads
Removed popout player button from sent link landing pages
Updated formatting of sent links so they show the name of the sender instead of just their email address.
Update Library Default View so that just the thumbnail is draggable to a playlist. Added the ability to select text.
User Admin: Filtering the users and then clicking on a column heading to sort no longer removes the filter.
User Admin Sorting users in the Admin panel no longer reveals the email address column
Updated copy on secondary filter
In the Group Administration, sort available users and groups alphabetically
Fixed - Unable to view media pages when Inbox is disabled
Download Playlist no longer returns a white screen and no download
Upload functionality fixed
Edit / New User - fixed Default Tab menu layout
Playlist XML Download - Filename downloaded now includes the playlist name
Create New User bug fixed when adding group membership
Add Quick-Search to New/Edit User
User Admin: Block Autofill on Password fields
Fixed - Markers "view more/less" displaying incorrectly
Persistent Marker Highlights on Media Page - Clicking on a marker a second time de-selects it now.
Fixed user admin group checkbox appears inactive
Fixed header image resizing on mobile
When 'Inbox' is disabled in the main System Preferences, users cannot load the Edit Profile page or the Shared Links page
Approved/Rejected/Archived states are now to be tied to the user, not a global state
Fixed Public Playlist Download Errors
Remove the &downloadvideo=1 from the url of a shared playlist link
Fixed Import Notification emails links
Public Playlist page: fixed thumbnails jumping around on mouse over
Playlist Download - removes source paths from Zip file
Fixed Import Notification Digest behavior
Send HighRes now appends to the log field in CatDV
Fixed iOS 7.1 Safari video layout issue
Fixed IE9 - Player controls don't show up properly
Fixed category Pulldowns aren't working consistently
Fixed playlist playback bugs
Fixed delete group bug - now when deleting a Group there is no ghost left in user settings for users that were in the group


text:
Turn Up to your 100 Mile Trail Event prepared
The aim of this 16 week plan is to teach you what to do every week to get your body in the best possible shape for your 100 Mile Ultra event.
Runners often enter an ultra, get over-excited, train too hard, get injured, or peak too early.
To avoid over doing it … or under doing it, the best way to approach your training is with a specific training plan that will help you to develop endurance and strength, and gradually increase volume, elevation, and intensity, so you peak at the right time for your 100 Mile event.
In 16 weeks from now, the aim of the plan is to have you feeling strong and prepared. Ready to really enjoy your ultra.
To give you an idea of what to expect, our beginner plan kicks off with a weekly volume of 50km and a long run of 20km. The advanced plan starts with a total weekly volume of 75km and a long run of 25km.
If these training volumes feel like a stretch right now, consider starting with our 100km trail plan instead. It’s better to build gradually than risk injury by doing too much too soon.
100 Mile Trail Training Load
3 / 5
3 / 5
4 / 5
4 / 5
What our members say
Frequently asked questions
Is this program suitable for a beginner?
The beginner level of this 21km plan is suitable for someone who has been running consistently for 3 -4 months and is comfortable running 8km – 10km regularly without injuries. You may be completely new to trail running and not used to running on hilly or technical terrain.
Can you help me train when I'm injured?
Probably, depending on your injury.rnWe are very sorry to hear you are injured. We know how difficult that can be.rnrnIf you have an injury that requires some rest from running, there are almost always some cross-training options available to help you maintain your fitness, strength, and sanity. By cross-training while you are injured, and including some simple strength, stretching and foam rolling routines, you will speed up your recovery time and ultimately make your return to running easier and more successful.rnrnYou can start our free 4 week cross training plan now for FREE.rnrnIt will help you to identify appropriate exercises based on your injury type and recommend what cross training methods will work best for your injury. When you are ready you can start our 10-week return to running program (url) or one of our run-specific rehab plans.
Can I start this program late?
You get immediate access to your training plan when you join. You can view the current week plus any previous weeks if you are training for a specific event.rnrnWe recommend that you start with a weekly volume that your body is currently able to cope with, which might mean starting on the beginner level before moving to intermediate a few weeks later.
About the founder
Physiotherapist and Elite Runner
Mark, has helped over 20,000 athletes improve their running and achieve their race goals. His holistic approach to training ensures noticeable improvements in your movement from the outset


title: Geographic farm

text:
Search as many properties as you want, with all the details you need. No matter how much or little information you have, you'll be able to track down the exact properties and owners you're looking for with PropertyRadar.
Mix, match and make hundreds of popular list templates to find exactly the leads most likely to need your services. PropertyRadar's list builder updates in real-time so you'll never miss another opportunity.
Harness the power of PropertyRadar, right in your pocket. Access property and owner data from the field when Driving For Dollars or Door Knocking to make important decisions faster.
Get everything you’d want to know about any property and owner. Access dozens of charts and infographics in PropertyRadar to better understand your leads and tailor your offers to be targeted, relevant, and neighborly.
Conduct property research in minutes with PropertyRadar. Customize comparables, access transaction history, and see neighborhood demographics to better qualify and close more deals.
Act quickly with important information right at your fingertips. With PropertyRadar, you can instantly access key risk indicators on ownership and affordability, and apply heat maps to see vacancy, turnover, and equity to understand your market.
Call leads directly from your computer or device with PropertyRadar. With built-in single-line and multi-line power dialers, you’ll be able to make up to 30% more calls.
Get your message to your leads, wherever they are. Run direct mail campaigns, and send email campaigns right in PropertyRadar.
Make doorknocking and field sales a breeze with progress tracking, note-taking, and automated follow-up. PropertyRadar helps you scale your in-person lead generation.
PropertyRadar integrates with over 5,000 software and service products to power the apps you’re already using.
Campaigns trigger off-property or owner criteria changes, so you can ensure your marketing is working even when you’re not.
Get instant mobile and email notifications, be the first to see new opportunities, and take action on them right from PropertyRadar.


title: Point to read ai story time plus pack

text:
AI Story Time helps develop LOVE for the Chinese language through stories.
-Choose the story kids love and watch it
-AI tutor reads the story in Chinese and explains its meaning in English and practice read aloud with immediate feedback
-Track number of stories read and compete with other kids
-Treasure and listen to your child's reading voice!
Log in on a computer using Chrome: story.betterchinese.com
Better Immersion Readers is a series of storybooks for young learners aged 5-9 with no prior exposure to Chinese. This series of 68 developmentally appropriate storybooks is designed with the vision to build learners’ day-to-day Chinese language communication skills from scratch, as well as to prepare them for future academic learning. As children learn best when the content is relevant and fun, the topics and storylines reflect their daily experiences, as well as the surroundings that they live in.
My First Chinese Words is for toddler and preschool learners with no prior exposure to Chinese. This set of 36 size-appropriate storybooks is designed with the idea that young children learn best when the information given is relevant to their everyday lives. The stories contain meaningful sentence structures, teaching students to communicate in full sentences as opposed to have them memorize individual characters.
Kit/Package Code: SP002018B
This Point to Read & AI Story Time Basic Pack includes:
1 Smart Reading Pen
1 set of Better Immersion Kindergarten Readers_Self (20 readers)
1 set of Better Immersion Kindergarten Readers_Relationships (16 readers)
1 set of Better Immersion Kindergarten Readers_Community (16 readers)
1 set of Better Immersion Kindergarten Readers_World (16 readers)
Sing With Better Chinese 36 songs ( audios built-in reading pen)
1-year full access to AI Story Time, App and Web
1 set of My First Chinese Words 36 storybook set


title: 1616

text:
New kitchens are expensive. A complete kitchen remodel can cost anywhere from $15,000 up to the mid-five-figures which is shocking! It’s better to look for cheaper and easier ways to make improvements to your existing kitchen to extend its useful life while giving it some much-needed beautification. Your wallet will thank you.
Here are four inexpensive and uncomplicated ways to make an older kitchen appear a bit more modern.
1. Repaint the Kitchen Cabinets
Many kitchen cabinets are ripe for repainting. It gets rid of ugly stains and paints over little insignificant chips on the doors too. Replacing the entire cabinetry is an expensive headache, but repainting is something you can do yourself over a weekend.
Choose a color that reflects rather than absorbs light. Yellow, orange or a similar hue brightens up the kitchen enormously. Use a primer for the drawers, door fronts and cabinet sides. Let the primer dry completely first. Then use a foam roller and a suitable brush to paint the kitchen cabinets properly.
Try a latex satin finish paint rather than something oil-based. It will look better and is less troublesome to use too. Take your time to produce a great final look.
2. Replace Pulls and Handles
With painted cabinets, you’ll want some new handles and pulls to really make the cabinets pop. Don’t try to re-fit the original ones as every mark and blemish will be obvious set against the backdrop of a satin finish paint job.
The handle design must fit in with the overall look of the kitchen. While some flare in the design choice is fine, don’t look for something too ornate because it will stand out for the wrong reasons. Handles and pulls must be practical first, and look good second – not the other way around. So, do not get too artsy with your selection.
3. Upgrade the Lighting
Changing the lighting can make a huge difference to an older kitchen. Quite often, the lighting hasn’t been upgraded since the kitchen was first installed. When adding new hanging lighting fixtures, light is directed downwards to the areas where it’s most needed. This can be low-hanging light fixtures, powerful LEDs or a row of spotlights.
Lighting up both the food preparation area, like an island in the kitchen, helps to avoid accidentally cutting yourself with a sharp knife because you couldn’t see clearly enough. Also, it’s difficult to cook well when you cannot clearly see when a piece of meat is cooked through or if the vegetables are being overcooked. Similarly, when it comes time to wash up or clean the kitchen, it’s useful to have better illumination there too.
4. Replace the Kitchenware
Food prep is just as important as the actual cooking, therefore it is important to have the right tools at your disposal. If your worktop appliances and utensils have seen better days, it can make a major difference to the look of your kitchen if you were to replace them all in a matching theme or color. This can include things like the toaster and kettle, a splash back or even worktop savers. If you are going to get wooden cutting boards, choose a classy wood finish such as walnut so that you can leave them out on display without them looking out of place.
When making your kitchen far more inviting as a place to hang out and cook for yourself (or your family) it often becomes a gathering spot. As an added bonus, you’ll also find yourself saving on restaurant and takeout expenses as you start to prefer the taste of your own cooking.


title: Dkny spring 2017 intimates film campaign starring emily ratajkowski

text:
Introducing the DKNY Spring 2017 intimates collection, sleepwear hosiery and sleepwear campaign, titled #GoodMorningDKNY, featuring actress and model Emily Ratajkowski. The campaign and film were shot by fashion photographer Sebastian Faena on location in DKNY’s native New York.
The film is set in a quintessential Manhattan apartment where Emily wakes, exiting her apartment to walk her dog, wearing nothing more than her DKNY bra and briefs. And- because anything can happen in New York- she steps onto the streets, greeting stunned neighbors, onlookers and viewers with, “Good Morning, New York.” The fashion film and campaign imagery, styled by Clare Richardson, will live online at DKNY.com and in iconic New York locations including The High Line, Times Square and in New York Subway stations. There will also be a comprehensive digital media plan across several fashion and lifestyle websites to support the launch.
“I have never been one to shy away from expressing my individuality – DKNY has always been about supporting strong, self-assured women and that is a message very important to me,” says Ratajkowski, “I’ve always been a fan of the brand and this intimates campaign and film are a natural fit.”
Emily embodies the attitude of a New Yorker – confident and not afraid to make a statement, her authentic charm feels inherent to DKNY’s DNA. Emily wears the black unlined demi bra and matching cheeky boyshort from the new classic lace collection. Additional styles worn throughout the campaign include Emily’s favorite sheer lace bralette and cheeky garter. The litewear collection appears as Emily lounges in bed wearing the ultra-smooth wirefree bralette and low rise bikini and with the mesh litewear bodysuit paired with the new fishnet thigh high in nude.
The DKNY intimates, hosiery and sleepwear spring collections are available at DKNY, Nordstrom, Lord + Taylor and Macy’s, both in stores and online, as well as select international retailers.


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Skip to code content (skip section selection)
(A) In the case of any discharge, including, but not limited to, accidental discharges, discharges of a non-routine, episodic nature, a non-customary batch discharge, or a slug load, which may cause potential problems for the system, the user shall immediately telephone and notify the city manager of the incident. This notification shall include the location of the discharge, type of waste, concentration and volume, if known, and corrective actions taken by the user.
(B) Within five days following such discharge, the user shall, unless this requirement is waived by the city manager, submit a detailed written report describing the cause of the discharge and the measures to be taken by the user to prevent similar future occurrences. Such notification shall not relieve the user of any expense, loss, damage, or other liability that may be incurred as a result of damage to the system, natural resources, or any other damage to person or property, nor shall such notification relieve the user of any fines, penalties, or other liability that may be imposed by this article or any other applicable provision of federal, State or local law.
(C) A notice shall be permanently posted on the user's bulletin board or other prominent place advising employees who to call in the event of a discharge described in subsection (A) above. Employers shall ensure that all employees, who may cause such a discharge to occur, are advised of the emergency notification procedure.
(D) Significant industrial users are required to notify the city manager immediately of any changes at its facility affecting the potential for a slug discharge.
(`64 Code, Sec. 25-54) (Ord. No. 2494, 2761)


title: Helpd 544

text:
Lithium iron phosphate batteries are characterized by high safety, high rate charge and discharge characteristics and long cycle life. The literature shows that when the charging condition is 1C rate charging to 3.65V, then the constant voltage is changed until the current drops to 0.02C, and then the 1C rate is discharged to a cut-off voltage of 2.0V. After 1600 cycles, the battery capacity still has 80% of the initial capacity. Lithium iron phosphate batteries also have good fast charging characteristics. Under the condition of 3C rate charging, they can be charged 55% in 15 minutes, and the capacity can exceed 95% in 30 minutes.
The biggest advantage of lithium iron phosphate battery is its safety. The chemical properties of lithium iron phosphate are stable, and the high temperature stability is good. It will start to decompose at 700-800℃, and it will not be exposed to impact, needle stick, short circuit, etc. It will release oxygen molecules, will not burn violently, and have high safety performance.
However, the disadvantage of lithium iron phosphate batteries is that their performance is greatly affected by temperature, especially in low-temperature environments, the discharge capacity and capacity will be greatly reduced. In addition, the energy density of lithium iron phosphate is relatively low. The weight energy density of the battery is only 120Wh/kg. If the energy density of the entire stack, including the battery management system, heat dissipation and other components is calculated, it is even lower.
In the field of small passenger cars, ternary lithium-ion battery packs have completely replaced lithium iron phosphate batteries. The ternary lithium ion battery pack has high energy density and better cycle performance than normal lithium cobalt oxide. At present, with the continuous improvement of the formula and the perfect structure, the nominal voltage of the battery has reached 3.7V, and the capacity has reached or exceeded the level of lithium cobalt oxide batteries.
Ternary lithium-ion battery refers to a lithium-ion battery that uses lithium nickel cobalt manganate as the positive electrode material and graphite as the negative electrode material. Unlike lithium iron phosphate, the ternary lithium-ion battery has a high voltage platform, which means that under the same volume or weight, the ternary lithium-ion battery pack has a greater specific energy and specific power. In addition, ternary lithium-ion batteries also have great advantages in terms of high-rate charging and low-temperature resistance.
The dispute over the ternary lithium-ion battery pack and lithium iron phosphate technical route seems huge, but for users and vehicle companies, the two technical routes actually have their own merits. In the final analysis, choosing different batteries for different models is the root of the problem.
The thermal stability of the ternary lithium-ion battery pack is poor, and it will decompose at 250-300℃. It will stop after encountering the combustible electrolyte and carbon materials in the battery. The heat that appears will further aggravate the decomposition of the positive electrode in a very short time. It will deflagrate inside. Although there are safety concerns, ternary lithium-ion battery packs have shown a trend to replace lithium iron phosphate batteries and become the mainstream of passenger cars because of the policy's energy density regulations.


title: Get started with linux containers in docker on wsl 2

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When Microsoft launched its Windows Subsystem for Linux (WSL) back in 2018, it was very clear why: It wanted to provide tools for developers building modern cloud applications. Microsoft needed a way to offer container development, with an eye on Azure-hosted distributed applications running on its Azure Kubernetes Service.
However, there were issues with the original WSL platform. It wasn’t easy to run Linux containers natively on Windows. Combined with ensuring WSL’s emulation layer could support Linux applications and services, it was clear that an emulation-based approach couldn’t meet the needs of most developers. That led to a redesign of the WSL platform and the resulting launch of WSL2 with a full Linux kernel earlier in 2019.
Microsoft’s WSL 2 previews are currently rolling out with Windows Insider 20H1 builds. As I noted in a previous article on WSL2, much of how it works is based on a collaboration between Microsoft and Docker engineers: LCOW (Linux Containers On Windows), which was intended to simplify running Linux containers directly on Windows. By using a hypervisor to host the relevant elements of the Linux OS and Docker platform, Windows development tools have access to the contents of the hosted containers. That thin-hypervisor approach now hosts the entire WSL environment, allowing Linux applications and containers to run natively on Windows systems.
Alongside the launch of WSL2, Docker announced a new version of its Docker Desktop for Windows, with a new version of the Docker application that would work with WSL2, supporting native Linux containers on Windows at long last.
That new WSL2 version of Docker Desktop is now available as a beta from Docker, requiring a Docker account. I signed up shortly after the new release was announced and last week finally got access to the new build. I’ve now installed it on my Fast Ring test PC, ready to try it out alongside Visual Studio Code’s new remote development tool as part of putting together an end-to-end development toolchain for Linux container applications.
Getting Docker running on WSL2
There are some prerequisites before running the new Docker Desktop. First, and most importantly, Docker Desktop expects Ubuntu to be your default WSL distribution, so use the wsl
command line tool to make sure that Ubuntu is first running in WSL2, and that it’s the default. If it’s running in WSL1, use the command wsl –set-version Ubuntu 2
to switch, and use wsl -l
to check that it’s default. To set it as default type wsl -s
. One important note: You need to be running Ubuntu 18.04, so either download it from the Windows Store directly or use sudo do-release-upgrade
to upgrade an existing Ubuntu WSL install to the latest release version.
With Ubuntu ready to go, you can now set up Docker Desktop. Once you’ve downloaded the preview Docker Desktop installer, run it. You’ll be prompted to choose whether to use Linux or Windows containers (with the option to change after installation). The default is to use Linux containers, so click OK. The installer then unpacks all the necessary files and sets up the appropriate mappings to WSL2’s file system. When the install is complete it’ll log you out of Windows, and should be ready to go once you log back in.
Running Docker Desktop with WSL2
When you launch Docker Desktop, there’s a new Docker icon in your Windows taskbar (if it’s not visible, check the notification area). Right-click on it and choose WSL Tech Preview to launch the Docker WSL2 installation tool. This checks for the correct Windows version, makes sure that you’re running Ubuntu as a WSL2 instance, and that it’s the default. If any prerequisites are missing, the installer will notify you what needs to be changed; you won’t be able to install the WSL 2 Docker tools until you’ve corrected the issue.
Once everything is ready, you can start the Docker Desktop and enable the WSL 2 Docker daemon. To check it’s running, open an Ubuntu terminal and check the Docker context that should have automatically been created, using docker context ls
. This should show that your default Docker instance is running in Ubuntu, with details of the current end point and the orchestrator being used. By default, the orchestrator is Docker’s swarm, as Kubernetes support is not yet available for WSL2 containers.
You can now start installing and running Docker containers from your own or from Docker’s own repository. A useful quick test is to download and run Docker’s own “hello-world” container from an Ubuntu WSL2 terminal: sudo docker run hello-world
. This will display a brief console message to show that it’s run successfully.
With Docker running on WSL2, you’re now ready to start building a full Linux development toolchain under Windows. I started with Visual Studio Code, which now offers remote development tooling that connects a Windows VS Code UI to debugging and file system access components running in Linux, in this case in WSL2’s Ubuntu. There’s no configuration required. From Visual Studio Code you open a remote connection to WSL2 and you have full access to the virtual hard drive that contains the WSL2 Linux file system, as well as using VS Code’s built-in terminal to access the WSL2 command line.
Building code on Windows for Linux containers
One of the interesting aspects of using WSL2 for container development is the ability to mix and match development environments. I’ve got the .Net command line tooling on my WSL2 test machine, so I used it to make a simple .Net Core MVC (Model-View-Controller) application in the Windows file system. From a VS Code terminal that’s running in WSL2 using remoting, I could then use an Ubuntu bash prompt to copy the .Net project from the Windows file system into the Linux file system, before creating an appropriate dockerfile and .dockerignore in VS Code and saving them directly into the WSL2 Linux file system.
With a dockerfile in place and a .Net Core application built and ready to go, all I needed to do was use the command line Docker tool to assemble the container, pulling in the appropriate images from Microsoft’s Docker repository to ensure that the .Net Core runtime was in place. Once Docker had created my application’s container, I was able to launch it, giving it a name and mapping it to an appropriate port. As recent builds of WSL2 have improved network address mapping from external applications it was easy enough to fire up a browser on my development system and connect to the resulting ASP.Net Core app running on Linux.
The combination of Docker for Windows and WSL2 certainly simplifies the process of building Linux container applications on a Windows development system. It’s not perfect, and there are still issues when crossing the boundaries between the two Windows personalities. However, the improved productivity, reduced overhead, and increased speed is worth the minor speed bumps. It’ll be interesting to watch how both Docker Desktop and WSL2 evolve in the next few months, though even in these early stages the combination is ready to use.


title: 28th july world hepatitis day

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World Hepatitis Day, observed on the 28th of July, serves as a crucial opportunity to strengthen national and global efforts in combatting hepatitis. The day aims to encourage individuals, partners, and the public to take action and engage in activities that raise awareness about the disease. It also highlights the urgent need for a more robust global response, as emphasized in the WHO’s Global Hepatitis Report of 2017.
The significance of this date stems from its association with the birthday of Dr. Baruch Blumberg, a Nobel Prize-winning scientist who made groundbreaking discoveries related to hepatitis B virus (HBV). His contributions include developing a diagnostic test and a vaccine for the virus.One of the most critical challenges to address in achieving the global elimination goals for hepatitis by 2030 is the low coverage of testing and treatment. Without adequate detection and care, the disease continues to have devastating consequences on individuals and communities worldwide.It is essential to understand that hepatitis is a silent threat.
Symptoms often manifest only in advanced stages of the disease. While there are multiple types of hepatitis viruses (A to E), hepatitis B and C are particularly concerning as they cause nearly 8000 new infections daily, most of which go undetected.This results in a staggering number of deaths, with over one million hepatitis-related deaths occurring every year, and one new chronic infection emerging every ten seconds. This is why maintaining liver health is fundamental to overall human well-being.The good news is that many hepatitis-related deaths can be prevented. Effective vaccines and treatments exist for hepatitis B, and there is even a cure for hepatitis C.
By taking necessary precautions and seeking appropriate medical care, individuals can protect themselves and their loved ones from the devastating impact of hepatitis. Remember, you only have one life and one liver; let’s work together to combat hepatitis and safeguard our health.
And from Delta Medical, we, along with our partners, will make every effort to ensure that modern medicines for this disease are accessible in all countries where we operate.


title: Finding love in a retirement home

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Finding love in a retirement home
June 23, 2014Some people say that love is a young person’s game but that’s utter nonsense! Love is something that transcends age, and even those in their twilight years can still find companionship, even if they’ve moved into a retirement home. In fact, a retirement home is a wonderful place to find love, and here’s why, along with some things to consider…
There are plenty of activities to take part in together
Any retirement home worth its salt will have a range of activities in which you can take part, and this is perfect to meet someone who has similar interests. Take the care homes run by Extra Care for instance: you can take part in loads of activities, including arts & crafts, gardening, woodwork, IT training, and many more social events and entertainment. After all, it’s important that you stay active when you’re older, and doing it in pairs or groups is much more enjoyable. Your social life won’t have been this active in years, so getting to know some great people (and maybe that special someone) is easy!
You’ll always have someone to talk to
Having good company is something that worries a lot of elderly people, but by finding a companion you will ensure you have some great company pretty much all of the time. Staying in a retirement home, you’ll never be too far from that person if you want some to chat to, or sometimes it’s just nice to sit with someone even if you don’t feel the need to talk.
Don’t go searching for it
As is always the case, love comes along when you least expect it, usually when you’re not looking for it. Don’t go storming in trying to find a potential partner or you’ll likely end up being rejected. You also have to be sensitive to others; you don’t know whether someone has lost a partner and isn’t looking for anyone else. Just let any potential relationships flourish naturally and the whole thing will be better for everyone.
Volunteers and staff are off limits!
It can be tempting when you see that hunky young staff member to develop a bit of a liking for them, but you need to remember that they’re off limits! A bit of harmless flirting never hurt anyone, however, so you might be able to get away with that!
Friendship rules all
When you’re in your elderly years, having a solid group of friends trumps everything, so don’t prioritise trying to find that one special person. You’ll meet loads of new people and will likely strike up some great friendships, which will be much more valuable. Love would be great but friendship rules all and is much easier to come by!


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Move Into Healing – A Journey Through Chair Yoga
When: Wednesdays (May 28th – July 2nd)
Time: 4:00 PM
Location: Mother’s Cupboard
61 Main St. S, Suite 1
Join us for a unique and empowering experience that blends gentle Chair Yoga with the exploration of healing modalities designed to support your body, mind, and spirit.
This class series is an invitation to slow down, connect inward, and learn powerful practices that you can carry with you beyond the classroom. Through a mindful structure that includes movement, breath, grounding, and reflection, we’ll explore over the next 6 weeks how intentional choices can lead to deeper well-being.
DROP INS WELCOME! You do not need to participate in every class to enjoy the benefits of each class offering.
✨ What to expect over the next 6 weeks
Chair Yoga – A gentle, accessible practice that supports strength, balance, and flexibility while staying rooted in comfort and safety.
Breathwork – Discover the power of your breath. Learn simple, effective techniques that activate your body’s natural healing response and calm the nervous system.
Grounding Practices – Reconnect to the earth and cultivate mindfulness. Learn how daily awareness of your environment can support your sense of calm and centeredness.
Stretching & Movement – Explore the relationship between body and brain. Through simple movements, gain insight into the internal systems that help regulate balance and well-being.
Internal Exercises – Gentle techniques to strengthen the body from the inside out, nurturing organs, circulation, and energetic flow.
Throughout each session, we’ll reflect on the idea that we are all on a journey, and that healing is a personal, powerful path.
You’ll be invited to consider:
“What do I want to choose for myself today?”
“How can I take charge of my health and healing?”
Whether you’re just beginning your wellness journey or looking to deepen it, this 6-week class offering will leave you with simple, supportive tools—and the inspiration to choose your path forward with curiosity and confidence.


title: Terrain Flex Japanese Techwear Hoodie

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Terrain Flex Japanese Techwear Hoodie
Step into contemporary streetwear with the Terrain Flex Japanese Techwear Hoodie, a perfect blend of functionality and cutting-edge design.
Crafted from lightweight, smooth fabric, this pullover combines comfort and practicality, making it ideal for both urban adventures and outdoor explorations.
The oversized silhouette offers a relaxed fit, ensuring all-day comfort while adding a bold, modern aesthetic to your wardrobe.
The deep forest green color exudes versatility, pairing effortlessly with a variety of styles and outfits.
Featuring wide, baggy sleeves, the design makes a fashion-forward statement while allowing ease of movement.
Equipped with large front flap pockets, the pullover is designed for ultimate functionality, letting you carry essentials with style.
The adjustable hood and high collar provide extra protection against the elements, while the asymmetrical hemline with drawstring adjustments ensures a customizable fit for every occasion.
The crossbody strap with an attached utility bag not only elevates its streetwear appeal but also adds unmatched practicality for carrying on-the-go essentials.
Layered detailing, with white fabric subtly peeking out from beneath, enhances the overall aesthetic, making this piece a standout in any setting.
Perfect for the fashion-conscious individual seeking a blend of utility and style, the Terrain Flex Japanese Techwear Hoodie redefines modern urbanwear.
Upgrade your wardrobe with this unique, functional, and stylish piece.
Whether you’re hitting the streets or embarking on an outdoor adventure, the Terrain Flex Japanese Techwear Hoodie is the ultimate statement piece you need.
- Machine Washing: If you opt for machine washing, select a gentle cycle with a mild spin and use cold water. Choose a mild detergent suitable for delicate fabrics. Place the item in a mesh laundry bag to safeguard the print and fabric during the wash cycle. Once the cycle is complete, remove the garment promptly to avoid creases.
- Hand Washing: Hand wash gently in cold water with a mild detergent to preserve the fabric's texture and color. Avoid harsh scrubbing, which can damage the delicate print.
- No Bleach: Do not use bleach, as it can significantly alter the colors and weaken the fabric fibers.
- Drying: After washing, press out the excess water. Do not wring or twist. Lay flat to dry in the shade. Avoid direct sunlight which can fade the colors.
- Ironing: If ironing is necessary, use a low heat setting and place a protective cloth between the iron and the garment to avoid damage to the prints.
- Storage: Store in a cool, dry place with adequate ventilation. Fold gently or hang on a padded hanger to maintain shape. Do not store with clips or pins attached to avoid fabric punctures.
We are dedicated to ensuring that your order reaches you swiftly and securely.
To achieve this, we have partnered with industry-leading couriers, including FedEx, UPS, and DHL, known for their reliable and efficient delivery services worldwide.
Once your order has been processed and is ready to leave our warehouse, you will receive a confirmation email with a tracking number.
With this number, you will be able to follow your parcel’s journey every step of the way.
Please anticipate the arrival of your purchase within 6-10 business days following its departure from our facility.
The estimated delivery timeframe accounts for the time required for customs clearance procedures for international orders, ensuring a hassle-free delivery to your doorstep.


title: The stepford wives a chilling exploration of domesticity and control

text:
maresiliencycenter.org – Bryan Forbes’ 1975 film, The Stepford Wives, is a chilling psychological thriller that delves into the darker side of suburban life. The film follows Joanna Eberhart (Nicole Kidman), a successful photographer who moves with her family to the idyllic town of Stepford, Connecticut. As she settles into her new life, Joanna begins to notice strange occurrences among the town’s women, who seem eerily perfect and submissive.
A Subversive Look at Gender Roles
The Stepford Wives is a subversive exploration of gender roles and societal expectations. The film critiques the idealized image of the perfect housewife, questioning the notion that women should conform to traditional roles and suppress their individuality. Joanna’s growing suspicion that the women of Stepford are not what they seem is a powerful indictment of the patriarchal structures that often limit women’s autonomy.
A Masterful Blend of Horror and Satire
The film masterfully blends elements of horror and satire, creating a disturbing yet humorous exploration of domesticity and conformity. The Stepford men, with their robotic and emotionless demeanor, are both frightening and farcical. The film’s underlying message about the dangers of conformity and the importance of individuality is both timely and thought-provoking.
A Timeless Tale of Female Empowerment
The Stepford Wives is a timeless tale of female empowerment and resistance. Joanna’s refusal to conform to the expectations of her new community is a powerful act of defiance. The film’s exploration of the tension between individuality and conformity continues to resonate with audiences today.
A Classic of Cinematic Horror
The Stepford Wives is a classic of cinematic horror, a film that has left an enduring legacy. Its exploration of the darker side of the American Dream and the dangers of conformity continues to inspire and unsettle audiences.


title: Indonesia aims for zero waste through indonesia bersih 2025 program

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The issue of waste is closely related to the increase in greenhouse gas emissions, which can lead to global warming. Therefore, the government is committed to reducing emissions by reducing waste in Indonesia through the concept of zero waste and zero emissions under the program titled “Indonesia Bersih 2025”.
The formation of methane gas from the process of decomposing organic waste and the release of carbon dioxide through waste incineration activities contribute to climate change on planet Earth.
The Ministry of Environment and Forestry has launched the “Indonesia Bersih 2025” program based on Presidential Regulation No. 97 of 2017 on the national policy and strategy for household waste and similar waste management.
Through this program, Indonesia aims to handle 70% of waste and reduce the remaining 30% through strategies from upstream to downstream.
The government is trying to raise awareness among households to handle their waste independently through composting and maggot cultivation for organic waste, as well as sorting and making handicrafts for non-biodegradable waste that is not easily decomposed.
According to data from the Ministry of Environment and Forestry, the majority of waste in Indonesia comes from households, with a composition of 43.3%, and food waste accounts for 41.1%.
In addition to its ambition to solve household waste, the government also obliges producers to reduce waste by redesigning product packaging to make it easy to recycle and to retrieve their non-biodegradable waste from society.
Furthermore, the downstream sector, which is located in the final processing site or TPA, uses various methods to maximize waste management and eliminate the need to dispose of waste at TPA.
Some of the technologies used include converting easily combustible and high-calorie waste such as plastic, paper, cloth, leather, and rubber into refuse-derived fuel or RDF.
RDF is a solid fuel substitute for coal used in power plants and cement factories. Waste management into RDF products can reduce the need for TPA land and improve environmental quality.
Several waste-to-energy power plants or PLTSa that are operating are located in Solo and Semarang in Central Java.
The PLTSa project is a collaboration program between the government and business actors to utilize energy from waste to reduce methane gas emissions by 30% by 2030 and achieve carbon neutrality by 2060.
Through various waste management and reduction strategies from upstream to downstream, the government hopes that there will be no more new TPA constructions by 2030, and Indonesia can be free from TPA starting in 2040.


title: Making the most of your ups battery resource

text:
Batteries are fundamental to the backup resource that a UPS system provides, yet they should also be considered as one of its weakest links. They have a finite useful working life and, if left in service long enough, will eventually need replacement. Their performance and operational life can also be compromised by fault conditions arising during service.
In this article, Ian Jackson, General Manager at KOHLER Uninterruptible Power, a KOHLER Company, looks at the UPS battery fault conditions that can arise, their causes, and how looking after your UPS batteries will benefit your power protection system.
Why Do You Need a Battery for Your UPS System?
All uninterruptible power supplies (UPSs) depend on a reliable energy storage mechanism for accumulating electrical energy during normal mains power supply and dispensing it to the critical load if this supply fails or exceeds critical limits. Currently, this function is most frequently fulfilled using VRLA lead acid batteries. Accordingly, in this article we look at the steps UPS owners and operators should take to optimise performance and maximise life for these batteries.
Modern sealed valve-regulated lead-acid (VRLA) batteries are environmentally friendly in operation, safe, and suitable for use in office environments. Manufacturers usually design their UPS cabinets to accommodate batteries, whilst also offering matching battery cabinets or racks for when extra battery autonomy is required.
What is a UPS Battery System?
A UPS battery system for a UPS application comprises of several blocks that can be built up and configured to meet specific needs of individual applications. Firstly, a single series string can be assembled to meet UPS float voltage settings, as the voltage across the string’s terminals equals the sum of the individual block voltages. Then, a number of series strings can be connected in parallel, either to increase the battery Ah rating and autonomy, or to build in battery redundancy. If one series string fails, any others connected in parallel will continue to operate.
Once the battery blocks have been configured and installed into the UPS system, a regular monitoring and maintenance programme should be initiated; this is vital to combine maximum battery performance with the longest possible operational life. To understand the necessity and benefits of such a programme, we can start by looking at the problems that can afflict UPS batteries during their working life.
What are the Common UPS Battery Problems?
Many problems arise from poor equalisation, where some battery blocks may overcharge while others are undercharging.
During overcharging, excessive charge voltages force a high overcharge current into the battery. This dissipates as heat, and in a VRLA battery, may cause gas emission through the safety valve. Within a short period this will corrode the positive plate material and shorten the battery’s life. Overcharging can also lead to batteries drying out and losing charging capacity, or suffering from thermal runaway. Thermal runaway occurs when a battery generates heat more quickly than it can dissipate it. This increases internal battery temperature, dries out the cells and possibly damages or ruptures the plastic battery container.
Undercharging can result in a battery block’s terminal voltage falling below its rated value. If a battery is left in a discharged state, or stored for a prolonged period of time, lead sulphate crystals start to form, acting as a barrier to recharging and preventing normal battery operation. Depending on the degree of sulphation, a battery may be recovered by constant current charging at a higher voltage than that used for standard charging, with the current limited to one tenth of the battery capacity, for no more than 4 to 10 hours.
Note that this process involves exceeding the battery’s normal recommended charging voltage, so the battery must be monitored at all times for safety’s sake. It must be removed from charge if excessive heat is generated. The voltage required to ‘force’ the maximum current into the battery will reduce as the battery recovers until normal charging can take place. However, in some circumstances a battery may never fully recover from sulphation, and will need to be replaced.
What are the Common UPS Battery Problems?
If batteries are insufficiently monitored, an undercharged condition may further deteriorate into a state of deep discharge. A battery is considered to be deeply discharged if its on-load voltage falls below a predetermined critical level. Severe sulphation will result, and in extreme cases the battery will be unable to accept a recharge and will need replacing.
How does Temperature Affect a UPS Battery’s Operation?
Battery operation is an electrochemical process and as such is highly temperature dependent. Most manufacturers recommend a working temperature environment of +20°C. As temperatures start to increase, more power can be extracted from the UPS battery. However long-term exposure to a higher temperature will adversely affect the anticipated service life of a battery. The empirical equation is that for every 10 °c above the recommended ambient temperature the life would be halved.
If the ambient temperature drops below optimum, the rate of chemical reaction and therefore battery performance reduces, although reduced temperatures have little effect on battery service life. Any AC component or ’ripple’ allowed to be superimposed onto the battery’s DC charging voltage will, however, adversely affect battery life. Modern transformerless design UPS systems utilise a dc:dc converter for the battery charger thereby eliminating the possible effects of this superimposed a.c. ripple associated with legacy transformer based design UPS units.


title: Yars

text:
The absence of a requirement for official Reddit API keys makes the tool very user-friendly and quick to set up.
The capability to extract a vast number of posts at once enables users to gather significant amounts of data for analysis efficiently.
The instruction to use rotating proxies helps users mitigate the risk of IP bans, enhancing the tool's usability in real-world scenarios.
While the tool is lightweight, comprehensive documentation would assist new users in maximizing its capabilities.
Improving the application's error handling could provide users with clearer insights when issues arise during the scraping process.
A more visually appealing user interface could attract users who prefer more aesthetics in their tools, particularly non-technical users.
Incorporating more advanced filtering options for data extraction could enhance usability and allow users to gather more relevant information.
UI & UX
Consider developing a simple web interface to visualize scraping options and results, making it more accessible to less technical users.
SEO or Marketing
Improving visibility through tutorials or blog posts on data scraping techniques using Yars may attract more users interested in data analysis.
Include multi-language support in the documentation to reach a broader audience beyond English speakers.
How do I install Yars?
Yars can be installed by cloning the GitHub repository and following the installation instructions provided in the README file.
What are rotating proxies and why do I need them?
Rotating proxies are a type of proxy server that changes your IP address at specific intervals. They are crucial when scraping data to avoid getting banned by websites, such as Reddit.
Can I alter the number of posts I scrape?
Yes, you can adjust the scrape limits; however, the maximum limit per request is capped at 2552 posts when scraping from 'r/all' on Reddit.


title: Renovo care group peter kinsey

text:
Reframe Cancer has announced Russell Young as its new commercial director.
Renovo Care Group: Peter Kinsey
Renovo Care Group has appointed Peter Kinsey as its chief executive, taking up the post on 1 March.
Renovo Care Group is an independent provider of assessment, treatment and rehabilitation for adults with neurological conditions including acquired brain injury (ABI) and progressive neurological disorders.
Kinsey has more than 35 years’ experience in the sector. He was an executive director in the NHS and worked as a local authority strategic commissioning manager. He later was appointed as chief executive at Care Management Group, a provider supporting people with learning disabilities. This company was sold to AMP Capital and Kinsey led the merger of the business with AMP-owned Regard Partnership to form Achieve together, a large learning disability provider.
After leaving Achieve together, Kinsey worked as a consultant helping organisations improve their ratings with the Care Quality Commission. For the past six months he has been interim chief executive of a care company supporting older people.
Renovo Care Group operates four services: Hollanden Park Hospital in Hildenborough, Kent, Swanborough House in Brighton, Victoria House in Farnborough and the new South Newton hospital near Salisbury.
The company is backed by investment firm Blantyre Capital, which stated it is looking for further investments in the healthcare sector to expand its business and presence in the sector.
Renovo’s current chief executive Philip Smith is stepping down, having overseen the initial phase of the creation of Renovo Care. Smith said: “My tenure as chief executive was meant to be short-term but, two years on, I am delighted to be handing the running of the business to Peter. His experience and skills will ensure Renovo Care is in good hands as it pursues ambitious growth plans
Kinsey said: “Philip has done a fantastic job securing the future of the services and of the facilities through the acquisition by Renovo Care. Renovo Care has exciting plans to expand its services and build on the quality care already offered and I am excited to be joining at such an interesting time and really looking forward to working with the team to achieve the company’s ambitions.”
Recruitment firm Compass Executives advised on Kinsey’s appointment.
Latest Executive Moves
The Association of Medical Insurers and Intermediaries (amii) has appointed Liam Kennedy as its vice-chair.


title: Keep risk running fundraiser

text:
Since August of 2009, RISK! has been operating on a budget of… well, almost nothing.
RISK! is a labor of love. We have a large team of people who each spend a tremendous amount of time making the RISK! podcast every week.
In addition to making the podcast, the RISK! team runs weekly free open story slams, produces 2-4 monthly live shows around the USA, runs a storytelling school, and does everything it can to keep a This American Life-style show running without a This American Life-style budget of any kind. We don’t have enough income to cover our payroll costs, let alone our additional costs for website maintenance, bandwidth for the podcast, publicity and marketing, touring, class space rental, and on and on and on. So now, we’re doing a public radio-style fundraiser here on IndieGoGo.com to keep RISK! running.
Until now, we’ve been getting by on favors, miracles, volunteers, a small amount of advertising income and one large donation from one of our biggest fans, but the till is almost empty, and the last thing we want to do is close shop.
Where The Money Goes
With your financial support, we can improve our recording equipment to give you a better-sounding show for a more pleasurable listening experience. We can put more effort into getting out on the road and bringing a live RISK! show to your hometown. We can stop worrying about how to pay our bills and get back to doing all we can to constantly improve our fans’ favorite show.
Ok, so what do I get if I give to RISK!?
The rewards you get for donating to RISK!’s fund raising campaign are one-of-a-kind gifts that you can’t get anywhere else, and that you’ll want to run out and tell all your friends about. Check out the all the rewards you can get for donations of anywhere from $10 to $10,000, and everywhere in between.


title: 1657

text:
The Institute of Communications Studies at the University of Leeds in the UK is holding a conference Monday, January 7, 2008 entitled: Broadcast News and the Active Citizen. I am thinking maybe David Hazinski, who I went to battle with over the value of Citizen Journalism, should be buying a ticket. Or better yet, Hazinski via the University of Georgia and I via Kennesaw State University, should sponsor a symposium and touch on many of the topics that will be discussed at Leeds, including
Broadcast news institutions are responding in unprecedented ways to a public which increasingly produces and consumes its own journalism.
These trends challenge traditional notions of citizenship and the role of broadcasters. While enabling exploration of a broad range of topics related to these phenomena, we seek to develop insights, in particular, into how the major news broadcasters of the UK – at the local and national levels – can adapt to an increasingly active, engaged, and demanding public.
The conference will explore the changing relationship between Broadcast News and Citizenship through a focus on three key themes.
- What does ‘broadcast news’ mean in the new media age?
- How has participatory journalism challenged notions of citizenship in the UK?
- How is communication now ‘mediated’ between traditional media and audiences/local communities/citizens?
Brian McNair, University of Strathclyde
Chris Atton, Napier University
Nick Couldry, Goldsmiths College – University of London
Vicky Taylor, BBC Interactive
Simon Bucks, Sky News
Mark Waddington, ITV Local
John Downing, Professor of International Communication, Southern Illinois University
Peter Horrocks, Head of BBC Newsroom
Thanks to my friend Francisco Seoane Pérez, a doctoral student at the University of Leeds, for the tip.


title: Elsa leggings

text:
Our new range of active performance leggings are suitable for any fitness activity from running, step classes, cycling... through to yoga or a gentle walk. They also look fantastic to wear as casualwear with a simple tee.
The beautiful elast print leggings show off a array of beautifully decorated elephants in bright and vibrant colours.
Our bespoke design, created specifically for the 3S Squad is super flattering with it's unique deep teal colour contrast side panel down each leg, creating a slimline leg silhouette.
These stunning shape enhancing, high waisted leggings have all the functionality you need.
High Spandex mix in the fabric, gives more flexibility and support
High quality inks used on the fabric
Squat proof… no see through pants here
Zipped back pocket
Side pocket (on leg) suitable for phone, bank card, gels
Two leg lengths available
Bright, bespoke designs
Tapered cut, no baggy knees or ankles
High waist with internal drawstring
Four way stretch fabric
Flat-lock seams for comfort and to reduce chafing
Available in two leg lengths Regular & 7/8
The fabric is very lightweight, with a smooth feel 80% Polyester, 20% Spandex. The fabric is quick drying, soft and with a four way stretch. Bonus - NO IRONING needed!
The fabric is lightweight & sensitive, please take care when wearing waist belts or water packs, or items with buckles or velcro type fastenings as these can all snag at the fabric and cause plucks and pulls.
Wash at 40 degrees
Suitable for handwashing
Do not tumble dry
Do not iron
Sizing Info - THESE ARE A SMALLER 3S RANGE WITH DIFFERENT SPANDEX MIX


title: Character Deck for Iron Nest Empire

text:
Character Deck for Iron Nest Empire
$5.00 Original price was: $5.00.$4.00Current price is: $4.00.
Set of 22 cards for “Legends of Signum 2: Warseer“ tabletop wargame.
Contains a complete list of the characters cards for “Iron Nest Empire”
This is a Character Card Set for the Iron Nest Empire faction in the tabletop game Legends of Signum 2: Warseer.
To allow players to use their existing character models from Legends of Signum in Legends of Signum 2: Warseer, we have released updated character cards for the new version of the game.
The Iron Nest Empire faction includes characters that previously belonged to the Holy Grypharim Empire faction and Fallen Nephilims faction.
You can find the full list of included character cards below. This list will continue to expand as we re-release cards for older characters in the future.
Full list of item in set: Read More...
Kiros the Midday Sun
Tankred, Candent Armours
Kerub the Armoured Griffin
Siliya the Emperor’s Jaegers Shooter
Astraea the Emperor’s Jaegers Shooter
Cordia the Emperor’s Jaeger Shooter
Brutus the Legionnaire
Lucius the Legionnaire
Cletus Aurelian, Legionnaire
Miretta, Knight of Aria’s Temple
Ariana, Knight of Aria’s Temple
Dahlia, Knight of Aria’s Temple
Aurora Corentyne the Sword of Aria
Rufus the Young Squire
Arcadia the Sunrise Knight
Cassandra, Celestial Healing
Herrick, “Burned Dogs”, 17th Infantry Regiment
Liobrenda the Temple Novice
Magnus the Red Inquisitor
Birthday Sale |
Orders placed between Monday and Friday are processed the same day and are usually dispatched in 1 - 3 business days of payment of an order.
All orders are shipped via Trackable First Class Airmail , unless otherwise selected.


title: Partnership Law Section 121-905

text:
Partnership Law Section 121-905
Surrender of certificate of authority
A foreign limited partnership may surrender its certificate of authority by filing with the department of state a certificate entitled, “Certificate of surrender of authority of.... (name of limited partnership)” signed by a general partner, or by a trustee, receiver or other person authorized by law to wind up such partnership. The authority of the foreign limited partnership to do business in this state shall terminate on such filing of the certificate of surrender of authority. A surrender shall not terminate the authority of the secretary of state to accept service of process on the foreign limited partnership with respect to causes of action arising out of doing business in this state.(b)
The certificate of surrender of authority shall state:(1)
the name of the foreign limited partnership as it appears on the index of names of existing domestic and authorized foreign limited partnerships of any type or kind in the department of state, and the fictitious name the foreign limited partnership has agreed to use in this state pursuant to § 121-902 (Application for authority, contents)section 121-902 of this article;(2)
the jurisdiction where it was organized;(3)
the date on which its certificate of authority to do business in this state was filed with the department of state;(4)
that it surrenders its authority to do business in this state;(5)
that it revokes the authority of its registered agent, if any, previously designated, and that it consents that process against it in any action or special proceeding based upon any liability or obligation incurred by it within this state before the filing of the certificate of surrender may be served on the secretary of state in the manner set forth in § 121-109 (Service of process on limited partnerships)section 121-109 of this article; and(6)
a post office address within or without this state to which the secretary of state shall mail a copy of any process against it served upon him or her. The limited partnership may include an email address to which the secretary of state shall email a notice of the fact that process against it has been electronically served upon him or her.
Section 121-905 — Surrender of certificate of authority, https://www.nysenate.gov/legislation/laws/PTR/121-905
(updated Jan. 6, 2023; accessed Jun. 9, 2025).


text:
A quick and efficient double encapsulation method for FACS-based droplet sorting
In this application note, we associate the double emulsion microfluidic platform with FACS sorting technology to sort out small droplets encapsulating fluorescent e-coli bacteria. Confining bacteria in small droplets is essential to perform single-cell analysis and to avoid fluorescence signal dispersion in bioassays based on protein or enzyme secretion.
Water-in-oil-in-water double emulsions efficiently play the role of both microbioreactor for efficient bacterial growth, and cellular activity and signal confinement, as the oil shell phase prevents fluorescence leakage.
Secoya developed and manufactured the RayDrop device used to perform this application note.
What is FACS sorting?
Analyzing cellular behavior at the single-cell level is important to life sciences as it enables a better understanding of cellular heterogeneity, which can be used for personalized medicine. FACS technology is a biology tool solution that offers an efficient selection of individual particles of interest among a complex population. FACS, or Fluorescence-Activated Cell Sorting, is based on the measurement of fluorescence signals at multiple wavelengths under laser excitation of cells flowing in line in a confined stream. The measured intensity gives information on the particle size, complexity, or specific phenotype, such as protein secretion. When the particle of interest is detected, an electric charge is applied, deflecting the cell towards a collecting tube.
Why is droplet sorting associated with droplet microfluidics?
Despite its potential for single-cell analysis and sorting, FACS sorting has two major limitations. First, it allows for single-cell analysis, but the analyzed sample usually is a bulk cell culture involving cellular interaction, preventing a real single-cell characterization (cells influence each other’s behavior). Second, FACS requires that the signal remains at the surface or inside the cells, which can be compatible with certain applications but often requires damaging cell treatment (i.e. fixation) and induces cellular death, preventing further analysis of the cell.
Droplet microfluidics removes these limitations. By confining single-cells inside droplets, droplet microfluidics allows for a full single-cell assay and ensures that the fluorescence signal stays in a closed area without using damaging chemicals. Droplet sorting using FACS technology recently emerged as a powerful tool to combine efficient sorting and single-cell analysis. Water-in-oil-in-water (W/O/W) double emulsions are of particular interest for droplet FACS sorting, since this application requires that the particles flow in an aqueous continuous phase .
In this application note, we encapsulate fluorescent bacteria in water-in-oil-in-water (W/O/W) double emulsions in one step using the FACS platform and perform droplet sorting using FACS technology to select only fluorescent particles.
This document was made in partnership with Delphine Lestrade, Head of the TWB (Toulouse White Biotechnology) cytometry platform, Sophie Lajus, researcher at the TBI (Toulouse Biotechnology Institute), and Sandra Pizzut-Serin and Sophie Bozonnet, from the ICEO-PICT platform.
As an expert in leading research and development (R&D) projects, TWB supports the industry via the development of innovative and sustainable solutions for the benefit of the planet and mankind.
TWB conducts R&D projects in the field of industrial biotechnology in collaboration with public laboratories and industrial players. They support the development of start-ups by offering them access to state-of-the-art scientific and technological environments and encourage the emergence of breakthrough innovations. By bringing together researchers, entrepreneurs, funders, institutions and industries, TWB integrates and leverages industry knowledge and simplifies the contractual relationship. This unique model accelerates the innovation process needed for the creation of an eco-responsible industry.
At TWB, the Cytometry Platform is responsible for developing and defining new protocols for high-throughput cytometry assays for the identification and characterization of microorganisms.
Delphine Lestrade, Head of the TWB (Toulouse White Biotechnology) cytometry platform
Webinar on double emulsion for FACS
Learn how to use our double emulsion platform to encapsulate bacteria in W/O/W DE and discover the DE sorting efficiency using FACS.
How to encapsulate bacteria in double emulsions
Making a double emulsion requires three different solutions: a core phase, a shell phase, and a continuous phase. The shell phase must be immiscible with the two other phases. Here, we add a fourth solution: the collection phase, in which we retrieve the emulsion for further droplet sorting. In addition, instead of having a simple core phase, we have a priming phase to start producing W/O/W DE, and an injection phase that contains biological materials (here bacteria in LB medium), that we introduce in the core fluid path using an injection loop.
Continuous phase: MilliQ water + 2% V/V Tween20
Shell phase: Dsurf 2% in HFE 7500 oil
Priming phase: MilliQ water for system priming and flow stabilization
Injection phase: LB culture medium with ~108/mL E.coli expressing the Green Fluorescent Protein (GFP) in the injection loop for bacterial encapsulation
Collecting phase: MilliQ water + 2% V/V Tween20 + 150mM NaCl
Real-Time Control & Lab Automation Software
OxyGEN – The new way to get full control of your microfluidic system and automate your lab setup.
How to encapsulate e-coli in double emulsions
We start the experiment by filling the Raydrop with the continuous phase. When the microfluidic chip is full, we set the pressure on the shell phase’s flow controller and make simple droplets of the shell. Once the flow is stabilized, we progressively increase the core flowrate until a double emulsion is formed. The double emulsion‘s shell thickness can be tuned by playing with the core and shell flow-rate ratio. In addition, the droplet size can be controlled by increasing or decreasing the continuous flow rate. At this stage, a simple W/O/W double emulsion is made.
We then make sure that the L-switch is set on position 2 and fill the injection loop by injecting the bacterial culture in port 5. Then, we switch to position 1, and after a certain time depending on the core phase flowrate, encapsulation of e-coli begins. After some time (15 – 25 minutes depending on the flowrate), the encapsulation is over.
For more details about the encapsulation process, please refer to the application note.
Before droplet sorting with FACS, particle size and monodispersity were assessed using microscopic imaging. The coefficient of variation of the core size was found to be 1.65% and the global droplet diameter has a coefficient of variation of 2%.
After microscopy validation, the double emulsions were passed through a Moflo Atrios FACS system for droplet sorting based on the fluorescence signal inside the drop. The fluorescent particles were collected in one tube and the non-fluorescent ones (empty or encapsulating non-fluorescent bacteria) in a second tube.
A microscopic validation was then performed on the content of both tubes. We notice that for the first vial, the droplets are all green florescent, whereas none of the droplets from tube 2 are. This confirms efficient droplet sorting. In addition, the fluorescence-activated cell sorting machine displayed a sorting efficiency of 75-95%.
FACS sorting was efficiently performed on double emulsions encapsulating Escherichia coli based on the measure of fluorescence inside the droplets. The W/O/W droplets generated with the platform showed good monodispersity, and the platform’s ability to generate high volumes of droplets in a short time with low biological sample consumption (200mL of double emulsion produced in <30min) was helpful for this application.
Workshop – Double emulsion production compatible with fluorescence-activated cell sorting (FACS) for cell sorting application
Read more- Microfluidic Application Notes
Double Emulsion Generation
Read more - Microfluidics White Papers
Droplet-based Microfluidics – A Complete Guide
Read more - Microfluidics White Papers
Double emulsion for the generation of microcapsules – a Review
Read more WEBINAR: Encapsulation of fluorescent bacteria in double emulsions for FACS sorting
K.K. Brower, C. Carswell-Crumpton, S. Klemm, B. Cruz, G. Kim, S.G.K. Calhoun, L. Nichols, P.M. Fordyce. Double emulsion flow cytometry with highthroughput single droplet isolation and nucleic acid recovery, LabChip, 2020, 20, 2062-2074
E. Mastrobattista, V. Taly, E. Chanudet, P. Treacy, B. T. Kelly, A. D. Griffiths, Chemistry & Biology 2005, 12, 1291.
DEWANDRE, Adrien, RIVERO-RODRIGUEZ, Javier, VITRY, Youen, SOBAC, Benjamin et SCHEID, Benoit, 2020. Microfluidic droplet generation based on non-embedded co-flow-focusing using 3D printed nozzle. Scientific Reports. 10 décembre 2020. Vol. 10, n° 1, pp. 21616. DOI 10.1038/s41598-020-77836-y.